1. ZN 9533 is a 49 clone synthetic cultivar. Parent clones trace to 37 populations selected for resistance to the following pests: bacterial wilt, Fusarium wilt, Verticillium wilt, Phytophthora root rot, anthracnose (race 1) and Aphanomyces (race 1) plus two additional isolates. Phenotypic recurrent selection was used Final selections were made from two-year old space plant selection nurseries near Livingston and Marshfield, Wisconsin and Napier, Iowa based on yield, winter survival, degree of leafhopper yellowing and stunting, freedom from leaf diseases, fall dormancy reaction and stem protein and digestibility.  Parentage traces to Synergy, ABI 9221, ABI 9223 and ABI 92PSL (8% each), ABI 92PLH, ABI 9131, ABI 9134 and ABI 9135 (7% each), Imperial, ABI 9021, Evolution, 620, Supercuts, ABI 9117A and Avalanche +Z (5% each) with 5% from numerous other sources.  Estimated % germplasm sources contributing to the genetic constitution of this variety. M. falcata Ladak M. varia Turkistan Flemish Chilean 4 7 23 19 31 8 Peruvian Indian African Arabian Unknown 6 0 0 0 2

2. ZN 9533 appears to be adapted to and is intended for use in the North Central Region of the U. S. It has been tested in Iowa, Wisconsin, Illinois and Minnesota.

3. Fall dormancy of ZN 9533 is similar to Ranger. Flower color of Syn 2 generation is approximately 69% purple and 30% variegated with a trace of cream, white and yellow. Winter survival is similar to Vernal.

4. ZN 9533 has high resistance to bacterial wilt, Fusarium wilt, Verticillium wilt, anthracnose (race 1), Phytophthora root rot and Aphanomyces (race 1) and moderate resistance to pea aphid. It has not been tested for reaction to blue alfalfa aphid, spotted alfalfa aphid, stem nematode and root knot nematode.

5. Seed increase is limited to one generation each of breeder (Syn 1), foundation (Syn 2) and certified (Syn 3) seed classes. Certified seed may be produced from either breeder or foundation classes. A 2,3 and 6year stand life is permitted on fields producing breeder, foundation and certified seed classes respectively. Foundation seed production is limited to the Pacific Northwest. Breeder seed was produced in 1995. ABI will maintain sufficient seed stocks for the life of the variety.

6. Certified seed will be available in 1998

7. Plant Variety Protection will not be applied for.

8. This information can be forwarded to the PVP office.

9. Variety Name: Date Submitted November 1997

Experimental Designations:ZN 9533.

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