ZN 9646

1. ZN 9646 was selected for resistance to the following pests: bacterial wilt, Fusariurn wilt,Verticillium wilt, Phytophthora root rot, anthracnose (race 1) and Aphanomyces (race 1) plustwo additional isolates. Phenotypic recurrent selection was used. Final selections were madefrom two-year old space plant selection nurseries near Napier, Iowa, Livingston and Marshfield, Wisconsin, based on yield, winter survival, degree of leafhopper yellowing and stunting,freedom from leaf diseases, fall dormancy reaction and stem protein.

Breeder seed (syn1) was produced in 1996 on approximately 25 cuttings of each clonetransplanted at random and harvested in bulk.

2. ZN 9646 appears to be adapted to the north central, east central and Great Plains regions ofthe U.S. It is intended for use in the north central, east central and Great Plains regions of theU.S. It has been tested in Iowa, Illinois, Indiana, Wisconsin, Pennsylvania, and Kansas.

3. Fall dormancy of ZN 9646 is similar to the FD 4 check Saranac. Flower color of syn2generation is approximately 72% purple and 28% variegated with a trace of cream, yellow andwhite.

4. ZN 9646 has high resistance to bacterial wilt, Verticillium wilt, Fusariurn wilt,anthracnose(race 1), Phytophthora root rot, pea aphid, and Aphanomyces (race 1). It hasmoderate resistance to blue alfalfa aphid. It has not been tested for reaction to spotted alfalfaaphid, stem nematode or root knot nematode.

5. Seed increase is limited to one generation each of breeder (syn1), foundation (syn2) andcertified (syn.3) seed classes. Certified seed may be produced from either breeder or foundationclasses. A one, three and six year stand life is permitted on fields producing breeder, foundationand certified seed classes respectively. Foundation seed production is limited to the Pacific Northwest. Breeder seed was produced in 1996. ABI will maintain sufficient seed stocks for thelife of the variety.

6. Certified seed will be available in 2000.

7. Application will not be made for Plant Variety Protection.

8. This information can be forwarded to the PVP office.

9. Date submitted: November 1999

Experimental designations: ZN 9646

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