W316

1. W316 is a 117-plant synthetic variety resulting from phenotypic recurrent selection for highforage quality. Parental germplasm traces to Trident 11, Pro-Cut 2, Ovation and Paramount. Approximate germplasm source contributions are M. falcata (16%), Ladak (17%), M. varia (22%), Turkistan (7%), Flemish (36%), and Chilean (2%).

2. W316 is adapted for use in the north central and east central regions of the United States. It isintended for use in the north central, east central and Great Plains regions. W316 has been yieldtested in Illinois, Minnesota, New York, and Wisconsin.

3. Flower color of W316 at syn2 approximates 100% purple with a trace of variegated and white. The fall dormancy of W316 is similar to 5246 (Class 3).

4. W316 has high resistance to anthracnose (race 1), bacterial wilt, Fusarium wilt, Phytophthora,root rot and Aphanomyces root rot (race 1); and resistance to Verticillium wilt, Aphanomycesroot rot (race 2), stem nematode, blue alfidfa aphid and pea aphid. Reaction to spotted alfalfaaphid and root knot nematode has not been adequately tested.

5. Breeder seed (syn1) was produced in 1996 on 117 plants under cage isolation at Warden,Washington. Sufficient foundation (syn ) seed will be produced for the expected life of thevariety and will be maintained by W-L Research. One generation of breeder (syn1) and twogenerations each of foundation (syn2 or 3) and certified (syn3 or 4) seed are recognized. Themaximum permitted length of stand for foundation and certified seed fields are three and five years, respectively. Foundation seed must be produced above 40 degrees North latitude.Production of syn3 foundation seed requires consent of the breeder.

6. Certified seed will be marketed in 1999.

7. It is undecided whether application will be made for Plant Variety Protection.

8. The information in this application can be turned over to the PVP office.

9. Experimental designation: W316

Date first accepted: January 1999

Date this amendment submitted: November 1999

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