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53H81 1. 53H81 is a 1 3 clone synthetic cultivar tracing to three Pioneer experimentals and one University of Minnesota germplasm. Parents were selected phenotypically for one or more of the following: resistance to Aphanomyces root rot, Phytophthora root rot, Verticillium Wilt, Fusarium Wilt, bacterial wilt, and potato leafhopper; field appearance, spring and fall growth. Parents were also selected through progeny testing for resistance to potato leafhopper, and field appearance in Platteville and Arlington, WI. Syn. 1 breeder seed was produced by intercrossing replicated stem cuttings of the 13 parent clones and bulking all seed 2. 53H81 is adapted to and intended for use primarily in the East central, and North central, regions of the United States. 53H81 has been tested in Hilbert, Arlington, WI; Princeton, IL and Chatham and Tavistock, Ontario, Canada 3. 53H81 is a dormant cultivar with an expected fall dormancy similar to FD-3 check. Flower color in the Syn. 2 generation is approximately 42% purple, 51% variegated and 7% yellow, with traces of white and cream 4. 53H81 is highly resistant to Aphanomyces root rot, anthracnose (race 1), Verticillium wilt, Fusarium wilt, bacterial wilt, and potato leafhopper; resistant to Phytophthora root rot, stem nematode and pea aphid; moderately resistant to spotted alfalfa aphid. Blue alfalfa aphid and root knot nematode were not tested. 5. Breeders seed (Syn 1) was produced on 103 plants (representing approximately equal numbers from each parent clone) in the greenhouse in Arlington, VY1 and bulked during the winter of 9798. Seed classes will be breeder, foundation (Syn 2 or 3), and certified (Syn 2, 3 or 4). Foundation seed may be produced from breeder or foundation. The second generation foundation seed may be produced at the discretion of Pioneer Hi-Bred International, Inc. Limitations of age of stand will be three and five years, respectively, for foundation and certified seed. 6. Certified seed will first be offered in the year 2000. 7. Application has been made for protection under the Plant Variety Protection Act and the certification option will not be requested. 8. As a means of added varietal protection, are you willing to have the information in this application turned over to the PVP office? Yes 9 Variety Name: 53H81 Date submitted: November27, 2000 Revised January 25, 2001 Experimental designations: Y53H81, W97CM81
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