Comparison of Alfalfa Produced by Hematoxylin Stain and Nutrient Culture Screening

Marihelen Kamp-Glass, David Powell and Guochen Yang. North Carolina A&T State University. 1601 E. Market St., Greensboro, NC 27411

Data for percent germination, percent development of mature plants and biomass data supporting the predictions of the screening processes are presented. Both screening methods yield similar results and match well with biomass data. These procedures, coupled with a multiple shoot organogenesis techniques, are currently being used to develop an aluminum tolerant alfalfa cultivar by breeding only those individuals identified as low pH and aluminum tolerant through seed screening.

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